Theory of dynamic light scattering software#
Theory of dynamic light scattering Offline#

Sensitive to contamination, e.g., from dust.Very sensitive to temperature, solvent viscosity, and refractive index.Cannot be used for particle sizes greater than 2Вµm.

Multiple light scattering when one particle is scattered by another before reaching the detector hinders accurate calculation of particle size.Low resolution in identification of closely-spaced multimodal size distributions.

Theory of dynamic light scattering Offline#

It is limited to offline and at-line methods of use.

The following limitations apply to DLS and direct imagining particle size analysers: With any analytical method, there exist several difficulties and limitations. This allows Eyecon 2 to more accurately calculate particle volume, which will affect the subsequently calculated D-Values. The cubed diameter of an equivalent circle fit used in many other image analysis systems. This approach results in a tighter volume fit than EyePASS uses a particle detection algorithm to identify and measure individual particles within the captured image. The application of the Mie theory can then be used to determine approximate particle volume and several other parameters.Д®yecon 2 identifies particles within a sample image which are then processed by EyePASS. The initial step of DLS is extracting the particle information and monitoring the change in the scattered intensity over time at a high temporal resolution, on the order of nano to a millisecond, and determine the correlation of the changing signal to

Theory of dynamic light scattering software#

The images captured are processed by the software EyePASSв„ў where the particle detection algorithm identifiesДЄnd measures individual particles within the image. Our in-house advanced particle size analyser, Eyecon 2,Д¬alculates particle size distributions, and shape information based on the measurement of individual particles within a sample image. Where various areas of bright and dark spots represent the constructive (bright spots) and destructive (dark spots) regions. If this process were to be imaged, a speckle pattern would be observed, Therefore, given the large light intensity associated with DLS setups, many photons can interfere both constructively and destructively due to the many possible scattering angles. When the scattered waves interference is constructive, the DLS detector records high light intensity and equally, when waves interference is destructive, the detector records low light intensity.ДЇigure: Constructive and Destructive Interference Waves, as is presented in the figure below. When two waves interact, they can combine in such a manner that the resultant wave is either an amplified or diminished superposition of the initially interacting

The fundamental principle governing the determination of particle size by DLS is interference. We have selected cutting-edge direct imaging particle size analysers, such as Eyecon 2, to explain the fundamentals of image analysis which will give you an appreciation of some of the factors to consider in your choice of particleДLS, sometimes referred to as Quasi-Elastic Light Scattering (QELS) and Photon Correlation Spectroscopy (PCS), is an optical method of determining particle size properties within a disperse sample by observing the change in scattered light intensity asДЄ function of time. We have created a detailed review of industry-standard methods such as Dynamic Light Scattering (DLS) and image analysis, where we contrast the principles of DLSДЄgainst image analysis. Exploring the particle size measurement landscape can be challenging and time-consuming.